human crc cell line colo 201  (Procell Inc)

Journal: Cancer Research and Treatment : Official Journal of Korean Cancer Association

Article Title: NFATC3 – PLA2G15 Fusion Transcript Identified by RNA Sequencing Promotes Tumor Invasion and Proliferation in Colorectal Cancer Cell Lines

doi: 10.4143/crt.2018.103

Figure Lengend Snippet: Identification of 6 fusion transcripts (FTs) in the colorectal cancer (CRC) cell lines. (A) Presence of PPP2CA – SKP1 , CTNNBIP1 – CLSTN1 , AKAP13 – PDE8A , NFATC3 – PLA2G15 , FZD2 – RPS24 , and KRT8 – PKM2 FTs was confirmed by reverse transcriptase–polymerase chain reaction (RT-PCR) in CRC cell lines. (B) The NFATC3 – PLA2G15 FT was detected in colon cancer tissues by RT-PCR. N, normal tissue; T, tumor tissue. (C) The NFATC3 – PLA2G15 FT was validated in the colo-320 cell line using Sanger-sequencing. The FT breakpoint was passed from exon 9 of NFATC3 to exon 2 of PLA2G15 . (D) NFATC3 – PLA2G15 FT mRNA expression was observed in the colo-320 fusion-positive cell line and the colo-205 fusion-negative cell line by RT-PCR. Forward primers targeted NFATC3 exon 4, 6, and 8 (black arrows) and reverse primers targeted PLA2G15 exon 2 (red arrow). Distilled water (DW) was used for the negative control.

Article Snippet: Human CRC cell lines (colo-201, colo-205, colo-320, HCT-15, HCT-116, HT-29, Lovo, LS174T, SNU-61, SNU-70, SNU-81, SNU-175, SNU-254, SNU-283, SNU-407, SNU-977, SNU-1033, SNU-1181, SNU-1235, SNU-1411, SNU-1544, SNU-1684, SNU-C1, SNU-C2A, SNU-C4, SNU-C5, SW-403, and SW-480) were purchased from the Korean Cell Line Bank [ ].

Techniques: Reverse Transcription, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Sequencing, Expressing, Negative Control